Network-Based Genomic Analysis of Human Oligodendrocyte Progenitor Differentiation.

ABSTRACT: Impaired human oligodendrocyte progenitor cell (hOPC) differentiation likely contributes to failed remyelination in multiple sclerosis. The characterization of molecular pathways that regulate hOPC differentiation will provide means to induce remyelination. In this study, we determined the gene expression profile of PDGFαR⁺ hOPCs during initial oligodendrocyte commitment. Weighted gene coexpression network analysis was used to define progenitor and differentiation-specific gene expression modules and functionally important hub genes. These modules were compared with rodent OPC and oligodendrocyte data to determine the extent of species conservation. These analyses identified G-protein β₄ (GNB4), which was associated with hOPC commitment. Lentiviral GNB4 overexpression

SUBMITTER: Pol SU 

PROVIDER: S-EPMC5550273 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature