Influence of Protein Scaffold on Side-Chain Transfer Free Energies.

ABSTRACT: The process by which membrane proteins fold involves the burial of side chains into lipid bilayers. Both structure and function of membrane proteins depend on the magnitudes of side-chain transfer free energies (ΔΔG_sc^o). In the absence of other interactions, ΔΔG_sc^o is an independent property describing the energetics of an isolated side chain in the bilayer. However, in reality, side chains are attached to the peptide backbone and surrounded by other side chains in the protein scaffold in biology, which may alter the apparent ΔΔG_sc^o. Previously we reported a whole protein water-to-bilayer hydrophobicity scale using the transmembrane β-barrel Escherichia coli OmpLA as a scaffold protein. To investigate how a different protein

SUBMITTER: Marx DC 

PROVIDER: S-EPMC5550287 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature