Application of genome-wide expression analysis to human health and disease.

ABSTRACT: The application of genome-wide expression analysis to a large-scale, multicentered program in critically ill patients poses a number of theoretical and technical challenges. We describe here an analytical and organizational approach to a systematic evaluation of the variance associated with genome-wide expression analysis specifically tailored to study human disease. We analyzed sources of variance in genome-wide expression analyses performed with commercial oligonucleotide arrays. In addition, variance in gene expression in human blood leukocytes caused by repeated sampling in the same subject, among different healthy subjects, among different leukocyte subpopulations, and the effect of traumatic injury, were also explored. We report that analytical variance caused by sample processing wa

SUBMITTER: Cobb JP 

PROVIDER: S-EPMC555033 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature