Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo.

ABSTRACT: Marrying synthetic biology with synthetic chemistry provides a powerful approach toward natural product diversification, combining the best of both worlds: expediency and synthetic capability of biogenic pathways and chemical diversity enabled by organic synthesis. Biosynthetic pathway engineering can be employed to insert a chemically orthogonal tag into a complex natural scaffold affording the possibility of site-selective modification without employing protecting group strategies. Here we show that, by installing a sufficiently reactive handle (e.g., a C-Br bond) and developing compatible mild aqueous chemistries, synchronous biosynthesis of the tagged metabolite and its subsequent chemical modification in living culture can be achieved. This approach can potentially enable many new app

SUBMITTER: Sharma SV 

PROVIDER: S-EPMC5550429 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature