A transcriptome atlas of rabbit revealed by PacBio single-molecule long-read sequencing.

ABSTRACT: It is widely acknowledged that transcriptional diversity largely contributes to biological regulation in eukaryotes. Since the advent of second-generation sequencing technologies, a large number of RNA sequencing studies have considerably improved our understanding of transcriptome complexity. However, it still remains a huge challenge for obtaining full-length transcripts because of difficulties in the short read-based assembly. In the present study we employ PacBio single-molecule long-read sequencing technology for whole-transcriptome profiling in rabbit (Oryctolagus cuniculus). We totally obtain 36,186 high-confidence transcripts from 14,474 genic loci, among which more than 23% of genic loci and 66% of isoforms have not been annotated yet within the current reference genome. Furthermo

SUBMITTER: Chen SY 

PROVIDER: S-EPMC5550469 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature