Single-cell profiling of lineage determining transcription factors in antigen-specific CD4⁺ T cells reveals unexpected complexity in recall responses during immune reconstitution.

ABSTRACT: Recent studies of protein and gene expression at the single-cell level have revealed that the memory T-cell compartment is more heterogeneous than previously acknowledged. Identifying different T helper subsets involved in memory responses at the single-cell level is thus necessary to understand the level of heterogeneity within this population. Antigen-specific CD4⁺ T cells were measured using the CD25/OX40 assay together with a qualitative multiplex single-cell RT-PCR assay. Transcription profiles and subset proportions within the antigen-specific CD4⁺ T-cell population were dissected. Cytomegalovirus (CMV)-specific CD4⁺ T-cell responses skewed toward a Th1 response, whereas Tetanus toxoid responses skewed toward a Th2 type response. Fluctuations in CD4

SUBMITTER: Phetsouphanh C 

PROVIDER: S-EPMC5550558 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature