Synergistic targeting of breast cancer stem-like cells by human γδ T cells and CD8⁺ T cells.

ABSTRACT: The inherent resistance of cancer stem cells (CSCs) to existing therapies has largely hampered the development of effective treatments for advanced malignancy. To help develop novel immunotherapy approaches that efficiently target CSCs, an experimental model allowing reliable distinction of CSCs and non-CSCs was set up to study their interaction with non-MHC-restricted γδ T cells and antigen-specific CD8⁺ T cells. Stable lines with characteristics of breast CSC-like cells were generated from ras-transformed human mammary epithelial (HMLER) cells as confirmed by their CD44^hi CD24^lo GD2⁺ phenotype, their mesenchymal morphology in culture and their capacity to form mammospheres under non-adherent conditions, as well as their potent tumorigenicity, s

SUBMITTER: Chen HC 

PROVIDER: S-EPMC5550559 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature