Mining sequence variations in representative polyploid sugarcane germplasm accessions.

ABSTRACT:

Background

Sugarcane (Saccharum spp.) is one of the most important economic crops because of its high sugar production and biofuel potential. Due to the high polyploid level and complex genome of sugarcane, it has been a huge challenge to investigate genomic sequence variations, which are critical for identifying alleles contributing to important agronomic traits. In order to mine the genetic variations in sugarcane, genotyping by sequencing (GBS), was used to genotype 14 representative Saccharum complex accessions. GBS is a method to generate a large number of markers, enabled by next generation sequencing (NGS) and the genome complexity reduction using restriction enzymes.

Results

To use GBS for high throughput genotyping highly polyploid sugarcane, the GBS analysis pipeli

SUBMITTER: Yang X 

PROVIDER: S-EPMC5551020 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature