Preparation and Bioactivity of Exopolysaccharide from an Endophytic Fungus Chaetomium sp. of the Medicinal Plant Gynostemma Pentaphylla.

ABSTRACT:

Background

Many exopolysaccharides from the endophytes in medicinal plants possess various potential bioactivities.

Materials and methods

The endophytic fungus JY25 was isolated from the leave of the Chinese medicinal plant Gynostemma pentaphylla and identified as Chaetomium sp. by its phylogenetic and physiological analysis. One exopolysaccharide (EPS) fraction was isolated from the fermentation broth by ethanol precipitation and purified by gel filtration chromatography on Sepharose CL-6B. The molecular characteristics were examined by GC-MS, FT-IR, and multiangle laser light scattering (MALLS).

Results

The monosaccharide composition analysis indicated that the purified EPS was mainly composed of glucose, mannose, arabinose, and galactose with the mole

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5551368 | biostudies-literature | 2017 Jul-Sep

REPOSITORIES: biostudies-literature