CD11a and CD49d enhance the detection of antigen-specific T cells following human vaccination.

ABSTRACT:

Background

Determining the efficacy of human vaccines that induce antigen-specific protective CD4 T cell responses against pathogens can be particularly challenging to evaluate. Surface expression of CD11a and CD49d has been shown to identify antigen-specific CD4 T cells against viral pathogens in mice. We hypothesized that CD11a and CD49d would also serve as markers of human antigen-specific T cells responding to vaccination.

Methods

A phase I vaccine trial enabled us to evaluate a novel gating strategy based on surface expression of CD11a and CD49d as a means of detecting antigen-specific, cytokine producing CD4 and CD8 T cells induced after vaccination of naïve individuals against leishmaniasis. Three study groups received LEISH-F3 recombinant protein combined with either

SUBMITTER: Christiaansen AF 

PROVIDER: S-EPMC5551405 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature