Predicting the response to CTLA-4 blockade by longitudinal noninvasive monitoring of CD8 T cells.

ABSTRACT: Immunotherapy using checkpoint-blocking antibodies against targets such as CTLA-4 and PD-1 can cure melanoma and non-small cell lung cancer in a subset of patients. The presence of CD8 T cells in the tumor correlates with improved survival. We show that immuno-positron emission tomography (immuno-PET) can visualize tumors by detecting infiltrating lymphocytes and, through longitudinal observation of individual animals, distinguish responding tumors from those that do not respond to therapy. We used ⁸⁹Zr-labeled PEGylated single-domain antibody fragments (VHHs) specific for CD8 to track the presence of intratumoral CD8⁺ T cells in the immunotherapy-susceptible B16 melanoma model in response to checkpoint blockade. A ⁸⁹Zr-labeled PEGylated anti-CD8 VHH

SUBMITTER: Rashidian M 

PROVIDER: S-EPMC5551571 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature