Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies.

ABSTRACT: Vaccines remain the most effective tool to prevent infectious diseases. Here, we introduce an in vitro booster vaccination approach that relies on antigen-dependent activation of human memory B cells in culture. This stimulation induces antigen-specific B cell proliferation, differentiation of B cells into plasma cells, and robust antibody secretion after a few days of culture. We validated this strategy using cells from healthy donors to retrieve human antibodies against tetanus toxoid and influenza hemagglutinin (HA) from H1N1 and newly emergent subtypes such as H5N1 and H7N9. Anti-HA antibodies were cross-reactive against multiple subtypes, and some showed neutralizing activity. Although these antibodies may have arisen as a result of previous influenza infection, we also obtained gp120

SUBMITTER: Sanjuan Nandin I 

PROVIDER: S-EPMC5551578 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature