Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Calcium Binding Properties and Allosteric Regulation of Downstream Regulatory Element Antagonist Modulator (DREAM).

ABSTRACT: Downstream regulatory element antagonist modulator (DREAM) is an EF-hand Ca²⁺-binding protein that also binds to a specific DNA sequence, downstream regulatory elements (DRE), and thereby regulates transcription in a calcium-dependent fashion. DREAM binds to DRE in the absence of Ca²⁺ but detaches from DRE under Ca²⁺ stimulation, allowing gene expression. The Ca²⁺ binding properties of DREAM and the consequences of the binding on protein structure are key to understanding the function of DREAM. Here we describe the application of hydrogen-deuterium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis to investigate the Ca²⁺ binding properties and the subsequent conformational changes of full-length DREAM. We demonstrate th

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5551683 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature