A module for Rac temporal signal integration revealed with optogenetics.

ABSTRACT: Sensory systems use adaptation to measure changes in signaling inputs rather than absolute levels of signaling inputs. Adaptation enables eukaryotic cells to directionally migrate over a large dynamic range of chemoattractant. Because of complex feedback interactions and redundancy, it has been difficult to define the portion or portions of eukaryotic chemotactic signaling networks that generate adaptation and identify the regulators of this process. In this study, we use a combination of optogenetic intracellular inputs, CRISPR-based knockouts, and pharmacological perturbations to probe the basis of neutrophil adaptation. We find that persistent, optogenetically driven phosphatidylinositol (3,4,5)-trisphosphate (PIP₃) production results in only transient activation of Rac, a ha

SUBMITTER: Graziano BR 

PROVIDER: S-EPMC5551696 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature