Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia.

ABSTRACT:

Objective

The etiopathogenesis and risk for development of biliary neoplasia in primary sclerosing cholangitis (PSC) are largely unknown. Microbes or their metabolites have been suggested to play a role. To explore this potential microbial involvement, we evaluated the differences in biliary microbiota in PSC patients at an early disease stage without previous endoscopic retrograde cholangiography (ERC) examinations, advanced disease stage, and with biliary dysplasia or cholangiocarcinoma.

Design

Bile samples from the common bile duct were collected from 46 controls and 80 patients with PSC during ERC (37 with early disease, 32 with advanced disease, and 11 with biliary dysplasia). DNA isolation, amplification, and Illumina MiSeq sequencing were performed for the V1-V3 regio

SUBMITTER: Pereira P 

PROVIDER: S-EPMC5552186 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature