High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii.

ABSTRACT: Microalga-based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability and sustainability. Secretion of recombinant proteins can accelerate the use of microalgal platforms by allowing post-translational modifications and easy recovery of products from the culture media. However, currently, the yields of secreted recombinant proteins are low, which hampers the commercial application of this strategy. This study aimed at expanding the genetic tools for enhancing secretion of recombinant proteins in Chlamydomonas reinhardtii, a widely used green microalga as a model organism and a potential industrial biotechnology platform. We demonstrated that the putative signal sequence from C. reinhardtii gametolysin can

SUBMITTER: Ramos-Martinez EM 

PROVIDER: S-EPMC5552477 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature