Metabolic networks for nitrogen utilization in Prevotella ruminicola 23.

ABSTRACT: Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut and oral cavity. To explore the metabolic networks for nitrogen assimilation in this bacterium, changes in gene expression profiles in response to variations in the available nitrogen source and to different concentrations of ammonium were analyzed by microarray and reverse transcription quantitative PCR, and linked with function by further proteomic analysis. The observed patterns of transcript abundances for genes involved in ammonium assimilation differed from the classical

SUBMITTER: Kim JN 

PROVIDER: S-EPMC5552732 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature