SNAP-25b-deficiency increases insulin secretion and changes spatiotemporal profile of Ca²⁺oscillations in β cell networks.

ABSTRACT: SNAP-25 is a protein of the core SNARE complex mediating stimulus-dependent release of insulin from pancreatic β cells. The protein exists as two alternatively spliced isoforms, SNAP-25a and SNAP-25b, differing in 9 out of 206 amino acids, yet their specific roles in pancreatic β cells remain unclear. We explored the effect of SNAP-25b-deficiency on glucose-stimulated insulin release in islets and found increased secretion both in vivo and in vitro. However, slow photo-release of caged Ca²⁺ in β cells within pancreatic slices showed no significant differences in Ca²⁺-sensitivity, amplitude or rate of exocytosis between SNAP-25b-deficient and wild-type littermates. Therefore, we next investigated if Ca²⁺ handling was affected in glucose-stimulated β cells us

SUBMITTER: Daraio T 

PROVIDER: S-EPMC5552776 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature