CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg^-/- Mice Results in Severe Immunodeficiency.

ABSTRACT: Immunodeficient mice engrafted with either normal or cancerous human cells are widely used in basic and translational research. In particular, NOD/SCID/IL2rg^-/- mice can support the growth of various types of human cancer cells. However, the hairs of these mice interfere with the observation and imaging of engrafted tissues. Therefore, novel hairless strains exhibiting comparable immunodeficiency would be beneficial. Recently, the CRISPR/Cas9 system has been used for efficient multiplexed genome editing. In the present study, we generated a novel strain of nude NOD/SCID/IL2rg^-/- (NSIN) mice by knocking out Foxn1 from NOD/SCID/IL2rg^-/- (NSI) mice using the CRISPR/Cas9 system. The NSIN mice were deficient in B, T, and NK cells and not only showed impaired T c

SUBMITTER: Wei X 

PROVIDER: S-EPMC5552779 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature