Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues.

ABSTRACT: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite class

SUBMITTER: Cacciatore S 

PROVIDER: S-EPMC5552883 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature