The choice of the DNA extraction method may influence the outcome of the soil microbial community structure analysis.

ABSTRACT: Metagenomics approaches and recent improvements in the next-generation sequencing methods, have become a method of choice in establishing a microbial population structure. Many commercial soil DNA extraction kits are available and due to their efficiency they are replacing traditional extraction protocols. However, differences in the physicochemical properties of soil samples require optimization of DNA extraction techniques for each sample separately. The aim of this study was to compare the efficiency, quality, and diversity of genetic material extracted with the use of commonly used kits. The comparative analysis of microbial community composition, displayed differences in microbial community structure depending on which kit was used. Statistical analysis indicated significant differenc

SUBMITTER: Zielinska S 

PROVIDER: S-EPMC5552907 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature