Farm level survey of spore-forming bacteria on four dairy farms in the Waikato region of New Zealand.

ABSTRACT: The aim of our study was to determine the occurrence and diversity of economically important spore-forming bacteria in New Zealand dairy farm systems. Farm dairy effluent (FDE) collected from Waikato dairy farms were tested for the presence of spore-forming bacteria, using a new culture-based methodology followed by genomic analysis. An enrichment step in which samples were inoculated in cooked meat glucose starch broth under anaerobic conditions, aided in the differential isolation of Bacillus and Clostridium species. Furthermore, the use of molecular methods such as ERIC genotyping, 16S rRNA gene sequence analysis identified different spore-forming bacteria present in FDE. C. sporogenes signature PCR gave further information on the phylogenetic relationship of the different Clostridium s

SUBMITTER: Gupta TB 

PROVIDER: S-EPMC5552919 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature