Illumina sequencing-based analysis of sediment bacteria community in different trophic status freshwater lakes.

ABSTRACT: Sediment bacterial community is the main driving force for nutrient cycling and energy transfer in aquatic ecosystem. A thorough understanding of the community's spatiotemporal variation is critical for us to understand the mechanisms of cycling and transfer. Here, we investigated the sediment bacterial community structures and their relations with environmental factors, using Lake Taihu as a model system to explore the dependence of biodiversity upon trophic level and seasonality. To combat the limitations of conventional techniques, we employed Illumina MiSeq Sequencing and LeFSe cladogram to obtain a more comprehensive view of the bacterial taxonomy and their variations of spatiotemporal distribution. The results uncovered a 1,000-fold increase in the total amount of sequences harvested

SUBMITTER: Wan Y 

PROVIDER: S-EPMC5552931 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature