Response of the rhizosphere prokaryotic community of barley (Hordeum vulgare L.) to elevated atmospheric CO₂ concentration in open-top chambers.

ABSTRACT: The effect of elevated atmospheric CO₂ concentration [CO₂ ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) from six chambers with ambient [CO₂ ] (approximately 400 ppm) and six chambers with elevated [CO₂ ] (700 ppm). The V4 region of the 16S rRNA gene was PCR-amplified from the extracted DNA and sequenced on an Illumina MiSeq instrument. Above-ground plant biomass was not affected by elevated [CO₂ ] at anthesis, but plants exposed to elevated [CO₂ ] had significantly higher grain yield. The composition of the rhizosph

SUBMITTER: Szoboszlay M 

PROVIDER: S-EPMC5552935 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature