Insights Into How Heme Reduction Potentials Modulate Enzymatic Activities of a Myoglobin-based Functional Oxidase.

ABSTRACT: Heme-copper oxidase (HCO) is a class of respiratory enzymes that use a heme-copper center to catalyze O₂ reduction to H₂ O. While heme reduction potential (E°') of different HCO types has been found to vary >500 mV, its impact on HCO activity remains poorly understood. Here, we use a set of myoglobin-based functional HCO models to investigate the mechanism by which heme E°' modulates oxidase activity. Rapid stopped-flow kinetic measurements show that increasing heme E°' by ca. 210 mV results in increases in electron transfer (ET) rates by 30-fold, rate of O₂ binding by 12-fold, O₂ dissociation by 35-fold, while decreasing O₂ affinity by 3-fold. Theoretical calculations reveal that E°' modulation has significant implications on electron

SUBMITTER: Bhagi-Damodaran A 

PROVIDER: S-EPMC5553066 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature