Proteomic mapping of p53 immunogenicity in pancreatic, ovarian, and breast cancers.

ABSTRACT:

Purpose

Mutations in TP53 induce autoantibody immune responses in a subset of cancer patients, which have been proposed as biomarkers for early detection. Here, we investigate the association of p53-specific autoantibodies with multiple tumor subtypes and determine the association with p53 mutation status and epitope specificity.

Experimental design

IgG p53 autoantibodies (p53-AAb), were quantified in 412 serum samples using a programmable ELISA assay from patients with serous ovarian, pancreatic adenocarcinoma, and breast cancer. To determine if patients generated mutation-specific autoantibodies we designed a panel of the most relevant 51 p53 point mutant proteins, to be displayed on custom programmable protein microarrays. To determine the epitope specificity we displayed

SUBMITTER: Katchman BA 

PROVIDER: S-EPMC5553208 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature