Characterization of Laminin Binding Integrin Internalization in Prostate Cancer Cells.

ABSTRACT: Laminin binding integrins α6 (CD49f) and α3 (CD49c) are persistently but differentially expressed in prostate cancer (PCa). Integrin internalization is an important determinant of their cell surface expression and function. Using flow cytometry, and first order kinetic modeling, we quantitated the intrinsic internalization rates of integrin subunits in a single cycle of internalization. In PCa cell line DU145, α6 integrin internalized with a rate constant (k_actual ) of 3.25 min^-1 , threefold faster than α3 integrin (1.0 min^-1 ), 1.5-fold faster than the vitronectin binding αv integrin (CD51) (2.2 min^-1 ), and significantly slower than the unrelated transferrin receptor (CD71) (15 min^-1 ). Silencing of α3 integrin protein expression in

SUBMITTER: Das L 

PROVIDER: S-EPMC5553695 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature