Prognostic significance of BRAF and NRAS mutations in melanoma: a German study from routine care.

ABSTRACT:

Background

Hotspot mutations of the oncogenes BRAF and NRAS are the most common genetic alterations in cutaneous melanoma. Specific inhibitors of BRAF and MEK have shown significant survival benefits in large phase III trials. However, the prognostic significance of BRAF and NRAS mutations outside of clinical trials remains unclear.

Methods

The mutational status of BRAF (exon 15) and NRAS (exon 2 and 3) was determined in melanoma samples of 217 patients with pyrosequencing and Sanger sequencing. The genotypes were correlated with clinical outcomes and pathologic features of the primary tumors. Time to disease progression was calculated with the cumulative incidence function. Survival analyses were performed with Kaplan-Meier estimates and Cox proportional hazards regression

SUBMITTER: Heppt MV 

PROVIDER: S-EPMC5553744 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature