The adaptive landscape of wildtype and glycosylation-deficient populations of the industrial yeast Pichia pastoris.

ABSTRACT:

Background

The effects of long-term environmental adaptation and the implications of major cellular malfunctions are still poorly understood for non-model but biotechnologically relevant species. In this study we performed a large-scale laboratory evolution experiment with 48 populations of the yeast Pichia pastoris in order to establish a general adaptive landscape upon long-term selection in several glucose-based growth environments. As a model for a cellular malfunction the implications of OCH1 mannosyltransferase knockout-mediated glycosylation-deficiency were analyzed.

Results

In-depth growth profiling of evolved populations revealed several instances of genotype-dependent growth trade-off/cross-benefit correlations in non-evolutionary growth conditions. On the genome l

SUBMITTER: Moser JW 

PROVIDER: S-EPMC5553748 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature