Non-lethal genotyping of Tribolium castaneum adults using genomic DNA extracted from wing tissue.

ABSTRACT: The red flour beetle Tribolium castaneum has become the second most important insect model organism and is frequently used in developmental biology, genetics and pest-associated research. Consequently, the methodological arsenal increases continuously, but many routinely applied techniques for Drosophila melanogaster and other insect species are still unavailable. For example, a protocol for non-lethal genotyping has not yet been adapted but is particularly useful when individuals with known genotypes are required for downstream experiments. In this study, we present a workflow for non-lethal genotyping of T. castaneum adults based on extracting genomic DNA from wing tissue. In detail, we describe a convenient procedure for wing dissection and a custom method for wing digestion that allows

SUBMITTER: Strobl F 

PROVIDER: S-EPMC5553768 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature