A comparison of Illumina and Ion Torrent sequencing platforms in the context of differential gene expression.

ABSTRACT:

Background

Though Illumina has largely dominated the RNA-Seq field, the simultaneous availability of Ion Torrent has left scientists wondering which platform is most effective for differential gene expression (DGE) analysis. Previous investigations of this question have typically used reference samples derived from cell lines and brain tissue, and do not involve biological variability. While these comparisons might inform studies of tissue-specific expression, marked by large-scale transcriptional differences, this is not the common use case.

Results

Here we employ a standard treatment/control experimental design, which enables us to evaluate these platforms in the context of the expression differences common in differential gene expression experiments. Specifically, we asse

SUBMITTER: Lahens NF 

PROVIDER: S-EPMC5553782 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature