Network design and analysis for multi-enzyme biocatalysis.

ABSTRACT:

Background

As more and more biological reaction data become available, the full exploration of the enzymatic potential for the synthesis of valuable products opens up exciting new opportunities but is becoming increasingly complex. The manual design of multi-step biosynthesis routes involving enzymes from different organisms is very challenging. To harness the full enzymatic potential, we developed a computational tool for the directed design of biosynthetic production pathways for multi-step catalysis with in vitro enzyme cascades, cell hydrolysates and permeabilized cells.

Results

We present a method which encompasses the reconstruction of a genome-scale pan-organism metabolic network, path-finding and the ranking of the resulting pathway candidates for proposing suitable

SUBMITTER: Blaß LK 

PROVIDER: S-EPMC5553788 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature