LSD1 dual function in mediating epigenetic corruption of the vitamin D signaling in prostate cancer.

ABSTRACT:

Background

Lysine-specific demethylase 1A (LSD1) is a key regulator of the androgen (AR) and estrogen receptors (ER), and LSD1 levels correlate with tumor aggressiveness. Here, we demonstrate that LSD1 regulates vitamin D receptor (VDR) activity and is a mediator of 1,25(OH)₂-D₃ (vitamin D) action in prostate cancer (PCa).

Methods

Athymic nude mice were xenografted with CWR22 cells and monitored weekly after testosterone pellet removal. Expression of LSD1 and VDR (IHC) were correlated with tumor growth using log-rank test. TRAMP tumors and prostates from wild-type (WT) mice were used to evaluate VDR and LSD1 expression via IHC and western blotting. The presence of VDR and LSD1 in the same transcriptional complex was evaluated via immunoprecipitation (IP

SUBMITTER: Battaglia S 

PROVIDER: S-EPMC5553900 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature