A curated genome-scale metabolic model of Bordetella pertussis metabolism.

ABSTRACT: The Gram-negative bacterium Bordetella pertussis is the causative agent of whooping cough, a serious respiratory infection causing hundreds of thousands of deaths annually worldwide. There are effective vaccines, but their production requires growing large quantities of B. pertussis. Unfortunately, B. pertussis has relatively slow growth in culture, with low biomass yields and variable growth characteristics. B. pertussis also requires a relatively expensive growth medium. We present a new, curated flux balance analysis-based model of B. pertussis metabolism. We enhance the model with an experimentally-determined biomass objective function, and we perform extensive manual curation. We test the model's predictions with a genome-wide screen for essential genes using a transposon-directed ins

SUBMITTER: Fyson N 

PROVIDER: S-EPMC5553986 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature