The Role of PGE₂ in Alveolar Epithelial and Lung Microvascular Endothelial Crosstalk.

ABSTRACT: Disruption of the blood-air barrier, which is formed by lung microvascular endothelial and alveolar epithelial cells, is a hallmark of acute lung injury. It was shown that alveolar epithelial cells release an unidentified soluble factor that enhances the barrier function of lung microvascular endothelial cells. In this study we reveal that primarily prostaglandin (PG) E₂ accounts for this endothelial barrier-promoting activity. Conditioned media from alveolar epithelial cells (primary ATI-like cells) collected from BALB/c mice and A549 cells increased the electrical resistance of pulmonary human microvascular endothelial cells, respectively. This effect was reversed by pretreating alveolar epithelial cells with a cyclooxygenase-2 inhibitor or by blockade of EP4 receptors on endo

SUBMITTER: Barnthaler T 

PROVIDER: S-EPMC5554158 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature