Lrp, a global regulator, regulates the virulence of Vibrio vulnificus.

ABSTRACT:

Background

An attenuated mutant (designated NY303) of Vibrio vulnificus, which causes serious wound infection and septicemia in humans, was isolated fortuitously from a clinical strain YJ016. This mutant was defective in cytotoxicity, migration on soft agar and virulence in the mouse. The purpose of this study was to map the mutation in this attenuated mutant and further explore how the gene thus identified is involved in virulence.

Methods

The whole genome sequence of mutant NY303 determined by next-generation sequencing was compared with that of strain YJ016 to map the mutations. By isolating and characterizing the specific gene-knockout mutants, the gene associated with the phenotype of mutant NY303 was identified. This gene encodes a global regulator, Lrp. A mutant, YH01

SUBMITTER: Ho YC 

PROVIDER: S-EPMC5554404 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature