Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.

ABSTRACT: Although baculovirus has been used as a safe and convenient gene delivery vector in mammalian cells, baculovirus-mediated transgene expression is less effective in various mammalian cell lines. Identification of the negative regulators in host cells is necessary to improve baculovirus-based expression systems. Here, we performed high-throughput shRNA library screening, targeting 176 antiviral innate immune genes, and identified 43 host restriction factor genes in a human A549 lung carcinoma cell line. Among them, suppression of receptor interaction protein kinase 1 (RIP1, also known as RIPK1) significantly increased baculoviral transgene expression without resulting in significant cell death. Silencing of RIP1 did not affect viral entry or cell viability, but it did inhibit nuclear translo

SUBMITTER: Wang CH 

PROVIDER: S-EPMC5554962 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature