Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells.

ABSTRACT: Cells and tissues can undergo a variety of biological and structural changes in response to mechanical forces. Only a few existing techniques are available for quantification of structural changes at high resolution in response to forces applied along different directions. 3D-magnetic twisting cytometry (3D-MTC) is a technique for applying local mechanical stresses to living cells. Here we describe a protocol for interfacing 3D-MTC with confocal fluorescence microscopy. In 3D-MTC, ferromagnetic beads are bound to the cell surface via surface receptors, followed by their magnetization in any desired direction. A magnetic twisting field in a different direction is then applied to generate rotational shear stresses in any desired direction. This protocol describes how to combine magnetic-fiel

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5555169 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature