Adductomics Pipeline for Untargeted Analysis of Modifications to Cys34 of Human Serum Albumin.

ABSTRACT: An important but understudied class of human exposures is comprised of reactive electrophiles that cannot be measured in vivo because they are short-lived. An avenue for assessing these meaningful exposures focuses on adducts from reactions with nucleophilic loci of blood proteins, particularly Cys34 of human serum albumin, which is the dominant scavenger of reactive electrophiles in serum. We developed an untargeted analytical scheme and bioinformatics pipeline for detecting, quantitating, and annotating Cys34 adducts in tryptic digests of human serum/plasma. The pipeline interrogates tandem mass spectra to find signatures of the Cys34-containing peptide, obtains accurate masses of putative adducts, quantitates adduct levels relative to a "housekeeping peptide", and annotates modification

SUBMITTER: Grigoryan H 

PROVIDER: S-EPMC5555296 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature