Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence.

ABSTRACT: Aging and oxidative stress are two prominent pathological mechanisms for Parkinson's disease (PD) that are strongly associated with the degeneration of dopamine (DA) neurons in the midbrain. DA and other catechols readily oxidize into highly reactive o-quinone species that are precursors of neuromelanin (NM) pigment and under pathological conditions can modify and damage macromolecules. The role of DA oxidation in PD pathogenesis remains unclear in part due to the lack of appropriate disease models and the absence of a simple method for the quantification of DA-derived oxidants. Here, we describe a rapid, simple, and reproducible method for the quantification of o-quinones in cells and tissues that relies on the near-infrared fluorescent properties of these species. Importantly, we demonst

SUBMITTER: Mazzulli JR 

PROVIDER: S-EPMC5555314 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature