A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae.

ABSTRACT:

Background

A single base pair mutation in the sodium channel confers knock-down resistance to pyrethroids in many insect species. Its occurrence in Anopheles mosquitoes may have important implications for malaria vector control especially considering the current trend for large scale pyrethroid-treated bednet programmes. Screening Anopheles gambiae populations for the kdr mutation has become one of the mainstays of programmes that monitor the development of insecticide resistance. The screening is commonly performed using a multiplex Polymerase Chain Reaction (PCR) which, since it is reliant on a single nucleotide polymorphism, can be unreliable. Here we present a reliable and potentially high throughput method for screening An. gambiae for the kdr mutation.

Methods

A Hot Li

SUBMITTER: Lynd A 

PROVIDER: S-EPMC555548 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature