Phenotypic Diagnosis of Lineage and Differentiation During Sake Yeast Breeding.

ABSTRACT: Sake yeast was developed exclusively in Japan. Its diversification during breeding remains largely uncharacterized. To evaluate the breeding processes of the sake lineage, we thoroughly investigated the phenotypes and differentiation of 27 sake yeast strains using high-dimensional, single-cell, morphological phenotyping. Although the genetic diversity of the sake yeast lineage is relatively low, its morphological diversity has expanded substantially compared to that of the Saccharomycescerevisiae species as a whole. Evaluation of the different types of breeding processes showed that the generation of hybrids (crossbreeding) has more profound effects on cell morphology than the isolation of mutants (mutation breeding). Analysis of phenotypic robustness revealed that some sake

SUBMITTER: Ohnuki S 

PROVIDER: S-EPMC5555484 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature