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Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.

ABSTRACT: Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge states, mitigating charge-state dependence that limits traditional approaches.

SUBMITTER: Riley NM 

PROVIDER: S-EPMC5555583 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.

Riley Nicholas M NM   Westphall Michael S MS   Coon Joshua J JJ  

Journal of proteome research 20170619 7

Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge state  ...[more]

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