Adipose Tissue Dendritic Cells Are Independent Contributors to Obesity-Induced Inflammation and Insulin Resistance.

ABSTRACT: Dynamic changes of adipose tissue leukocytes, including adipose tissue macrophage (ATM) and adipose tissue dendritic cells (ATDCs), contribute to obesity-induced inflammation and metabolic disease. However, clear discrimination between ATDC and ATM in adipose tissue has limited progress in the field of immunometabolism. In this study, we use CD64 to distinguish ATM and ATDC, and investigated the temporal and functional changes in these myeloid populations during obesity. Flow cytometry and immunostaining demonstrated that the definition of ATM as F4/80⁺CD11b⁺ cells overlaps with other leukocytes and that CD45⁺CD64⁺ is specific for ATM. The expression of core dendritic cell genes was enriched in CD11c⁺CD64^- cells (ATDC), wher

SUBMITTER: Cho KW 

PROVIDER: S-EPMC5555636 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature