Unravelling the interplay of sphingolipids and TGF-β signaling in the human corneal stroma.

ABSTRACT:

Purpose

To delineate the role of Sphingolipids (SPLs) in the human cornea and their cross-talks with transforming growth factor beta (TGF-β) in order to develop novel, non-invasive therapies.

Methods

Human corneal fibroblasts (HCFs) were harvested from healthy donors, stimulated with Vitamin C to promote extracellular matrix assembly, treated with exogenous sphingosine-1-phosphate (S1P) or sphingosine kinase inhibitor 2 (SPHK I2) and isolated after 4 weeks for further analysis.

Results

Data showed that S1P led to a significant decrease in cellular migration where SPHK I2 just delayed it for 24h. Significant modulation of the sphingolipid pathway was also noted. Sphingosine kinase-1 (SphK1) was significantly downregulated upon exogenous stimulation with S1P at a concen

SUBMITTER: Nicholas SE 

PROVIDER: S-EPMC5555661 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature