Formation of friable embryogenic callus in cassava is enhanced under conditions of reduced nitrate, potassium and phosphate.

ABSTRACT: Agrobacterium-mediated transformation is an important research tool for the genetic improvement of cassava. The induction of friable embryogenic callus (FEC) is considered as a key step in cassava transformation. In the present study, the media composition was optimized for enhancing the FEC induction, and the effect of the optimized medium on gene expression was evaluated. In relative comparison to MS medium, results demonstrated that using a medium with reducing nutrition (a 10-fold less concentration of nitrogen, potassium, and phosphate), the increased amount of vitamin B1 (10 mg/L) and the use of picrolam led to reprogram non-FEC to FEC. Gene expression analyses revealed that FEC on modified media increased the expression of genes related to the regulation of polysaccharide biosynthes

SUBMITTER: Utsumi Y 

PROVIDER: S-EPMC5555663 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature