Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells.

ABSTRACT: The process of human cardiac development can be faithfully recapitulated in a culture dish with human pluripotent stem cells, where the impact of environmental stressors can be evaluated. The consequences of ionizing radiation exposure on human cardiac differentiation are largely unknown. In this study, human-induced pluripotent stem cell cultures (hiPSCs) were subjected to an external beam of 3.7 MeV α-particles at low mean absorbed doses of 0.5, 3, and 10 cGy. Subsequently, the hiPSCs were differentiated into beating cardiac myocytes (hiPSC-CMs). Pluripotent and cardiac markers and morphology did not reveal differences between the irradiated and nonirradiated groups. While cell number was not affected during CM differentiation, cell number of differentiated CMs was severely reduce

SUBMITTER: Baljinnyam E 

PROVIDER: S-EPMC5555881 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature