Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils.

ABSTRACT: Voltage-gated proton channels (Hv1/VSOP) are expressed in various cells types, including phagocytes, and are involved in diverse physiological processes. Although hvcn1, the gene encoding Hv1, has been identified across a wide range of species, most of the knowledge about its physiological function and expression profile is limited to mammals. In this study, we investigated the basic properties of DrHv1, the Hv1 ortholog in zebrafish (Danio rerio) which is an excellent animal model owing to the transparency, as well as its functional expression in native cells. Electrophysiological analysis using a heterologous expression system confirmed the properties of a voltage-gated proton channel are conserved in DrHv1 with differences in threshold and activation kinetics as compared t

SUBMITTER: Ratanayotha A 

PROVIDER: S-EPMC5555884 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature