Genetic diversity of Fasciola spp. isolates from northern part of Iran: comparison with southwestern isolates.

ABSTRACT: Animal and human fascioliasis is a health and economic problem in few of tropical and subtropical areas of the world, including Iran. The present study aimed to determine the genotype diversity of Fasciola isolates in different hosts from Gilan province, northern Iran, and compare it with those isolates from southwestern Iran. Forty-eight adult Fasciola spp. were collected from cattle, sheep, and goats from slaughterhouse in Talesh, north of Iran. DNA was extracted from each fluke and PCR-RFLP was used to find out the species of the isolates. The ribosomal ITS1 and ITS2, and mitochondrial genes of NDI and COI from individual Fasciola isolates of each host were PCR-amplified and the PCR products were sequenced. Genetic variation within and between the isolates was evalu

SUBMITTER: Sarkari B 

PROVIDER: S-EPMC5555931 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature