Antitumor properties of Coenzyme Q₀ against human ovarian carcinoma cells via induction of ROS-mediated apoptosis and cytoprotective autophagy.

ABSTRACT: Coenzyme Q₀ (CoQ₀, 2,3-dimethoxy-5-methyl-1,4-benzoquinone) has been reported to exert anticancer properties against human breast/lung cancer cells. This study investigated the in vitro and in vivo anticancer properties of CoQ₀ on human ovarian carcinoma (SKOV-3) cells and xenografted nude mice, and revealed the underlying molecular mechanism. CoQ₀ induced G₂/M arrest through downregulation of cyclin B1/A and CDK1/K2 expressions. CoQ₀-induced autophagy as a survival mechanism was evidenced by increased accumulation of LC3-II, GFP-LC3 puncta, AVOs formation and Beclin-1/Bcl-2 dysregulation. Increased TUNEL-positive cells and Annexin-V/PI stained cells indicated CoQ₀-induced late apoptosis. Both mitochondrial (caspa

SUBMITTER: Hseu YC 

PROVIDER: S-EPMC5556069 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature